Coding

Part:BBa_K106010:Design

Designed by: Willis Wong   Group: iGEM08_UCSF   (2008-10-23)


PhyB, Aar1 AB part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 490
    Illegal BamHI site found at 572
    Illegal XhoI site found at 523
    Illegal XhoI site found at 542
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 739


Design Notes

As a BD part, there is no ATG, and the stop codon is included. For more information on AarI cloning, please refer to the UCSF 2008 iGEM page. Fuses only at the N-terminal only.


Source

Arabidopsis thaliana

References